MCF-7 Whole Cell Lysate

Artikelnummer: ROC-W09-000-360
Artikelname: MCF-7 Whole Cell Lysate
Artikelnummer: ROC-W09-000-360
Hersteller Artikelnummer: W09-000-360
Alternativnummer: ROC-W09-000-360
Hersteller: Rockland Immunochemicals
Kategorie: Zellen/Zellkultur
Applikation: SDS-PAGE, WB
Konjugation: Unconjugated
Alternative Synonym: MCF-7 Whole Cell Lysate, MCF7 WCL
Konzentration: 1mg/mL by BCA assay
Puffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle: Human
Formulierung: Liquid
Application Verdünnung: WB: User Optimized
Anwendungsbeschreibung: W09-000-360 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 20 µl of Human MCF-7 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under reducing conditions (lane 1). Molecular weight standards are shown in lane 2.
Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.