1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle:
Human
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
WB: User Optimized
Anwendungsbeschreibung:
W09-001-372 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.
Coomassie stained SDS-PAGE of 30 µg of Human Derived A549 Whole Cell Lysate (Ready-to-Use) resolved in a 4-20% gradient gel under non-reducing conditions (lane 2). Molecular weight standards are shown in lane 1.
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