1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle:
Human
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
WB: User Optimized
Anwendungsbeschreibung:
W09-001-GX2 has been tested by SDS-PAGE and Western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Western Blot Results of Rabbit Anti-SQSTM1/p62 Antibody using U-87 MG Lysate. Western Blot of Rabbit Anti-SQSTM1/p62 Antibody. Lane 1: Opal Prestain Molecular Weight Marker (p/n MB-210-0500). Lane 2: U-87 MG whole cell lysate. Load: 35µg lysate per lane. Primary Antibody: Anti-SQSTM1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 60 mins at RT. Block: 5% BLOTTO/TBST (p/n B501-0500). Predicted MW: ~48 kDa. Observed MW: ~45, 60kDa. Protein undergoes acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation.
SDS-PAGE Results of U-87 MG Whole Cell Lysate. Lane 1: U-87 MG Whole Cell Lysate (10µg) Reduced. Lane 2: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 3: U-87 MG Whole Cell Lysate (10µg) Non-Reduced. Results show wide range of molecular weight bands with no signs of degradation.
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