Fluorescein Diphosphate Tetraammonium Salt (FDP), CAS [[217305-49-2]]
Artikelnummer:
USB-257686
Hersteller Artikelnummer:
257686
Alternativnummer:
USB-257686-5
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Upon interaction with phosphatases the colorless and non-fluorescent FDP is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments that are equipped with the Argon laser excitation. Alternatively, FDP can also be used to detect phosphatases in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In some literature, FDP was considered to be one of the most sensitive fluorogenic phosphatase substrates. FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases. FDP is thermally unstable, and special cautions need be excised for storing the solid sample and stock solutions. Synonyms: FDP CAS Number: 217305-49-2 Molecular Formula: C20H26N4O11P2 Molecular Weight: 560.39 Appearance: Yellow solid Purity: 98% Excitation: 498nm Emission: 517nm Absorbance: 487nm Extinction Coefficient (cm-1 M-1): 80000 Quantum Yield: 0.7900 Correction Factor (260nm): 0.32 Correction Factor (280nm): 0.275 Solubility: Water Storage and Stability: Store at -20C under inert atmosphere. Light sensitive. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molekulargewicht:
560.39
Reinheit:
98%
Formulierung:
Yellow solid
CAS Nummer:
[217305-49-2]
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