STK3, also known as serine/threonine-protein kinase 3, is a mammalian Ste20-related protein kinases most closely related to Drosophila Hippo, a major regulator of cell proliferation and survival during development. It is a serine/threonine kinase that functions early in a pheromone responsive signal transduction cascade in yeast. It activates the human large tumor suppressor kinase Lats1. It modulates stress-induced cardiac hypertrophy. Source: Recombinant protein corresponding to aa1-322 from human STK3, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~37.6kD (331aa), 40-57kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: ADPMEQPPAP KSKLKKLSED SLTKQPEEVF DVLEKLGEGS YGSVFKAIHK ESGQVVAIKQ VPVESDLQEI IKEISIMQQC DSPYVVKYYG SYFKNTDLWI VMEYCGAGSV SDIIRLRNKT LIEDEIATIL KSTLKGLEYL HFMRKIHRDI KAGNILLNTE GHAKLADFGV AGQLTDTMAK RNTVIGTPFW MAPEVIQEIG YNCVADIWSL GITSIEMAEG KPPYADIHPM RAIFMIPTNP PPTFRKPELW SDDFTDFVKK CLVKNPEQRA TATQLLQHPF IKNAKPVSIL RDLITEAMEI KAKRHEEQQR ELEEEEENSD EDELDHHHHH H Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
