TPI1, Recombinant, Human, aa1-249, His-Tag (Triosephosphate Isomerase, TPI)
Artikelnummer:
USB-377992
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| Artikelname: | TPI1, Recombinant, Human, aa1-249, His-Tag (Triosephosphate Isomerase, TPI) |
| Artikelnummer: | USB-377992 |
| Hersteller Artikelnummer: | 377992 |
| Alternativnummer: | USB-377992-20,USB-377992-100 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Source: Recombinant protein corresponding to aa1-249 from human TPI1 protein, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~28.8kD (269aa), confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >3000units/mg, in which one unit will convert 1umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate/minute at pH 7.5 at 25C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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