UMPS, also known as uridine 5-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU. Source: Recombinant protein corresponding to aa1-480 from human UMPS, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~53kD (486aa) 50-70kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
