Human CD221 is a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): 1:10-1:40. Protein digestion is required Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Pancreas, placenta Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: BALB/c mice were fused with cells of the SP2/0 myeloma cell line Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
