Applications: Suitable for use in suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase Electron Microscopy, Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against anti-peroxidase, anti-rat IgG and anti-rat serum.
Formulierung:
Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamycin sulfate Labeled with horseradish peroxidase (HRP).
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