Recombinant protein corresponding to human MIP-3 beta, expressed in E. coli.
MIP-3 beta acts as a chemoattractant for T and B lymphocytes, dendritic cells, NK cells and myeloid progenitor cells, through specific binding to CCR7, a G-protein coupled receptor known to be up-regulated by Epstein-Barr virus infected B cells and Herpes virus infected T cells. A correlation between the response to MIP-3 beta and the level of CCR7 expression and has been revealed in the maturation of dendritic cells (DCs), and increased levels of both MIP-3 beta and CCR7 expression have been shown in bronchoalveolar lavage fluid (BALF) and BALF cells from patients with the granulomatous disorder sarcoidosis, associated predominantly with CD4+ T lymphocyte alveolitis, and hence T cell recruitment. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (sandwich): 0.25-1ug/ml. (100ul/well) in conjunction with M1202-66 as a capture antibody, allows the detection of at least 0.2- 0.4ng/well of recombinant human MIP-3beta. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3beta is 1.5-3ug/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
