PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)
Artikelnummer:
USB-P4278-01
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| Artikelname: | PLTP, Recombinant, Mouse (Phospholipid Transfer Protein) |
| Artikelnummer: | USB-P4278-01 |
| Hersteller Artikelnummer: | P4278-01 |
| Alternativnummer: | USB-P4278-01-25 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Mouse PLTP Phospholipid transfer protein (PLTP, also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6). Source: A DNA sequence encoding the mature mouse PLTP (Glu18-Ala493, AAH03782) was fused with a 6X-His tag at the C-terminus and expressed in a mouse myeloma cell line, NS0. Molecular Mass: Based on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells. Storage and Stability: 12 months from date of receipt, 20 to 70C as supplied. 1 month, 2 to 8C under sterile conditions after reconstitution. 3 months, 20 to 70C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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