Wisteria floribunda lectin is a 116,000 to 125,000 molecular weight glycoprotein with an isoelectric point of pH 5.4. Two subunits of about 60,000 daltons are produced in SDS gels, dissociating into 4 chains of about 30,000 daltons in the presence of reducing agents. The binding specificity is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked alpha or beta to the 3 or 6 position of galactose. Used to fractionate lymphocyte populations, the lectin although not mitogenic, elicits the production of lymphokines from murine splenocytes (references available upon request). Inhibiting/Eluting Sugar: 200mM N-acetylgalactosamine Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
116125
Reinheit:
Homogeneous by SDS-PAGE
Formulierung:
Supplied as a lyophilized powder. Reconstitute with 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Do not vortex.
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