The Nuclear and Cytoplasmic Protein Extraction Kit is designed for the rapid and efficient isolation of highly enriched cytoplasmic and nuclear protein fractions from eukaryotic samples, including cultured cells and fresh tissues. The kit provides a simple and reliable workflow that enables separation of cytoplasmic and nuclear proteins in approximately 90 minutes. Using a sequential extraction strategy, cytoplasmic proteins are released under hypotonic conditions through selective cell membrane disruption, followed by centrifugation to pellet intact nuclei. Nuclear proteins are then extracted using a high-salt nuclear extraction reagent to ensure efficient solubilization. The resulting protein fractions are suitable for direct use in downstream applications such as Western blotting, EMSA, DNase footprinting, reporter gene analysis, and enzyme activity assays. The kit supports up to 100 samples and allows reagent volumes to be adjusted according to sample type and input amount.
Samples:
Cultured cells and freshly isolated eukaryotic tissues.
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