Anti-Bundibugyo Ebolavirus, GP (Clone BDBV-255)-Purified No Carrier Protein, Clone: [BDBV-255], Monoclonal

Article Name:	Anti-Bundibugyo Ebolavirus, GP (Clone BDBV-255)-Purified No Carrier Protein, Clone: [BDBV-255], Monoclonal
Biozol Catalog Number:	ABI-12-8195
Supplier Catalog Number:	12-8195
Alternative Catalog Number:	ABI-12-8195-250UG
Manufacturer:	Abeomics
Category:	Antikörper

Specificity: BDBV-255 activity is directed against an epitope near the base of Bundibugyo ebolavirus (BDBV) glycoprotein. Antigen Distribution: Ebola virus glycoprotein is a surface protein expressed on the virus envelope. Background: Ebola virus is a member of the Filoviridae family that causes severe disease in humans with a mortality rate of 25-90%1. Three Ebola species are responsible for lethal outbreaks: Zaire ebolavirus (EBOV), Bundibugyo ebolavirus (BDBV), and Sudan ebolavirus (SUDV). The Ebola virus envelope contains a single surface glycoprotein (GP) which is responsible for viral attachment to the host cell, endosomal entry, and membrane fusion1. GP is composed of two subunits, GP1 and GP2. GP1 has a heavily glycosylated mucin-like domain and a glycan cap. GP2 contains the internal fusion loop, transmembrane domain, and stalk. GP is the major target of neutralizing monoclonal antibody (mAb) and vaccine design against Ebola virus. mAbs targeting GP1 are capable of neutralizing all known filovirus GPs2,3. A pan-Ebola virus mAb is highly desirable to protect against future outbreaks. BDBV-255 is a GP mAb isolated from B cells of a survivor of the 2007 Uganda BDBV outbreak3. Peripheral blood mononuclear cells from the survivor were transformed with Epstein-Barr virus, CpG, and additional supplements. Subsequently, cell supernatants were screened by ELISA for binding to GPs from BDBV, EBOV, or MARV filoviruses. Positive cells were fused with HMMA2.5 myeloma cells by electrofusion and cloned by single-cell fluorescence-activated cell sorting. In binding and neutralization assays, BDBV-255 binds to and neutralizes BDBV but not other filoviruses3. BDBV-255 targets an epitope that extends below the base of GP, possibly within the membrane-proximal external region (MPER) of GP2, in a region that is likely not conserved between filoviruses. Stable binding may require the full MPER, transmembrane domain regions, and a membrane. One GP trimer is able to accommodate three BDBV-255 antibodies.

Clonality:	Monoclonal
Clone Designation:	[BDBV-255]
Purity:	Purity: >=90% monomer by analytical SEC and SDS-Page Preparation: Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Target:	Bundibugyo Ebolavirus

Application Dilute:

B, ELISA, EM, FA, N