T7 RNA polymerase is a T7 promoter-specific, DNA-dependent, 53 RNA polymerase from T7 bacteriophage. Using double stranded DNA as the template, it transcribes RNA complementary to the single stranded DNA located at the downstream of T7 promoter. T7 RNA polymerase has been commonly used for in vitro mRNA synthesis.
Buffer:
100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 1 mM EDTA, 20 mM 2-mercaptoethanol, 0.1% Triton X-100, 50% (v/v) Glycerol.
Purity:
95%
Form:
Liquid
Application Dilute:
Single stranded RNA synthesisRNA probe synthesissiRNA precursor synthesisPrecursor for RNA splicing preparationCapped RNA synthesisThe optimal working dilution should be determined by the end user.
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