The polymerase is produced in E. coli. As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 RNA polymerase can be used for in vitro transcription to obtain more synthetic products.
Buffer:
50 mM Trizma base, 100 mM NaCl, 1mM EDTA, 20 mM beta-ME, 50% (v/v) Glycerol, 0.1% Triton X-100, pH 7.5.
Purity:
95%
Form:
Liquid
Application Dilute:
Single stranded RNA synthesisRNA probe synthesissiRNA precursor synthesisPrecursor for RNA splicing preparationCapped RNA synthesisThe optimal working dilution should be determined by the end user.
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