Ribonuclease R (RNase R) is a Mg2+-dependent 3-5 exonuclease. RNA can be cleaved into dinucleotide and trinucleotide gradually from the 3-5 direction by RNase R. RNase R can digest most of the linear RNA. However, it is difficult to digest circular RNA, lariat RNA and short double-stranded RNA molecules with less than 7 nucleotides of 3 end protrusion.
Buffer:
50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1% Triton X-100, 0.1 mM EDTA, 50% Glycerol (pH7.5 at 25C).
Form:
Liquid
Application Dilute:
Gene expression studiesAlternative splicing studyEnrichment of circRNA from biological samplesRecognition of intronic lariat RNAIdentification of exonic circRNA.The optimal working dilution should be determined by the end user.
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