E. coli Poly (A) Polymerase does not depend on the presence of template, and can catalyze the sequential incorporation of ATP in the form of AMP into the 3´ terminal of RNA, i.e., the addition of polyA tail to the 3´ terminal of RNA.
Buffer:
20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.1%TritonX-100, 50% (v/v) Glycerol, pH 8.0.
Form:
Liquid
Application Dilute:
For RNA 3´ end labeling.Poly(A) tails are added to RNA for cloning or affinity purification. For example, Poly (A) was added to miRNA to provide oligo-dT primer binding sites for cDNA synthesis.Improve the efficiency of RNA translation in eukaryot
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