T4 RNA Ligase is an ATP-dependent double-strand RNA ligase (dsRNA ligase). It can be used for intramolecular cyclic ligation and intermolecular linear ligation of double-stranded RNA, and can also be used for intramolecular or intermolecular RNA ligation of double-stranded nucleic acids (RNA duplex, RNA/DNA hybrid strand, or DNA duplex) for the 3 hydroxyl group of the RNA strand and the 5 phosphate group of the DNA strand.
Buffer:
10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5.
Purity:
95%
Form:
Liquid
Application Dilute:
Ligation reaction of nicks in double-stranded RNAThe ligation of the 3 hydroxyl group of RNA and the 5 phosphate group of DNA in the double-stranded structureDouble-stranded RNA undergoes intramolecular cyclization and intermolecular linear liga
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