mRNA Cap 2´-O-Methyltransferase can add a methyl group to the 2´-O of the first nucleotide of the Cap0 structure, [m7GpppN1(pN)x-OH(3)], next to the RNA 5´ end. Methylation of capped mRNA from Cap 0 to Cap1 naturally exists in eukaryocyte, where the Cap1 structure shows an important effect for translation, thus for expression as well. This product doesnt show a reactivity to RNA substrate other than RNA with Cap0 structure.
Buffer:
20 mM Tris-HCl pH8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% TritonX-100, 50% (v/v) Glycerol.
Purity:
95%
Form:
Liquid
Application Dilute:
Methylate the 2´-O of the first nucleotide of the Cap0 structure, to realize mRNA translation and expression.The optimal working dilution should be determined by the end user.
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