Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. BsaI (E. coli) is expressed from E. coli using gene recombination technology, our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contamination.
Buffer:
10 mM Tris-HCl, 300mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/mL HAS, 50% Glycerol, pH 7.4 at 25C.
Form:
Liquid
Application Dilute:
Molecular cloning.GenotypingSouthern hybridizationRestrictive fragment length polymorphism (RFLP)The optimal working dilution should be determined by the end user.
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