Produced in BALB/c mice using highly pure recombinant Human MIP-3beta as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3beta is 2.0-4.0 ng/lane, under reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MIP-3beta when used with BioGems biotinylated antigen affinity purified anti-Human MIP-3beta (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.. Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. . Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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