Produced in BALB/c x c57BL/6 F1 mice using highly pure (>98%)recombinant human NT-4 as the immunizing antigen. This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human NT-4 when used with BioGems biotinylated antigen affinity purified anti-human NT-4 (60-253BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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