Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Anti-Rat SCF specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect rat SCF by sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat SCF (62-073BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.. Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of rat SCF (50.0 ng/ml), a concentration of 1.2-1.8 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted
