Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography employing immobilized Human beta-NGF matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Human beta-NGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human beta-NGF (60-247BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary.. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted
