Anti-Human sTNF Receptor Type II Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ABT-ABG10527-U100
Supplier Catalog Number:
ABG10527-U100
Alternative Catalog Number:
ABT-ABG10527-U100-100UG
Manufacturer:
Abcepta
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, WB
Species Reactivity:
Human
Clonality:
Polyclonal
Purity:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Anti-Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hsTNF-receptor II by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human sTNF-receptor II (60-328BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.. Immunohistochemistry: This antibody stained PC-3 and CaCo-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm.. Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of Human sTNF Receptor-II (0.5 µg/ml) a concentration of 15.0 - 20.0 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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