Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2. Anti-Human TFF-2 specific antibody was purified by affinity chromatography employing an immobilized Human TFF-2 matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TFF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Human TFF-2 by sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human TFF-2 (60-307BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TFF-2.. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.25mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.<br /> <span style=font-size: 10px,><br /> Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. <p class=MsoNormal><span style=font-size: 12pt,><o:p></o:p>. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted
