Flow Cytometry, Optimal dilutions should be determined by end users.
Flow Cytometry analysis of RH35 cells using anti-DSG2 antibody (A02035). Overlay histogram showing RH35 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035&44, 1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U87 cells using anti-DSG2 antibody (A02035). Overlay histogram showing U87 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035&44, 1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of HEPA 1-6 cells using anti-DSG2 antibody (A02035). Overlay histogram showing HEPA 1-6 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035&44, 1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x10
IHC analysis of DSG2 using anti-DSG2 antibody (A02035). DSG2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0&44, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of DSG2 using anti-DSG2 antibody (A02035). DSG2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0&44, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of DSG2 using anti-DSG2 antibody (A02035). DSG2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0&44, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
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