E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).
Alternative Names:
DDX4, DDX4, VASA, DEAD box protein 4, VASA, Vasa homolog
Boster Bio Anti-DDX4/MVH Antibody Picoband catalog A02448. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Western blot, 0.25-0.5µg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Immunofluorescence, 5µg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human
Flow Cytometry analysis of PC-3 cells using anti-DDX4 antibody (A02448). Overlay histogram showing PC-3 cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448&44, 1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of DDX4 using anti-DDX4 antibody (A02448). DDX4 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0&44, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4 Antibody (A02448) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of DDX4 using anti-DDX4 antibody (A02448). DDX4 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0&44, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4 Antibody (A02448) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
Western blot analysis of DDX4 using anti-DDX4 antibody (A02448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX4 antigen affinity purified polyclonal antibody (Catalog A02448) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for DDX4 at approximately 79 kDa. The expected band size for DDX4 is at 79 kDa.
Flow Cytometry analysis of SiHa cells using anti-DDX4 antibody (A02448). Overlay histogram showing SiHa cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448&44, 1µg/1x106 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&44, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of DDX4 using anti-DDX4 antibody (A02448). DDX4 was detected i
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