TSPAN8 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB100339
- Images (6)
| Article Name: | TSPAN8 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB100339 |
| Supplier Catalog Number: | orb100339 |
| Alternative Catalog Number: | BYT-ORB100339-50,BYT-ORB100339-100,BYT-ORB100339-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human TSPAN8 (101-200/237aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CO-029, TM4SF3, TSN8_HUMAN, TSPAN8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029, |
| TSPAN8 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 26 kDa |
| UniProt: | P19075 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TSPAN8 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (orb100339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (orb100339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TM4SF3) Polyclonal Antibody, Unconjugated (orb100339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (orb100339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (orb100339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Pancreas tissue lysates, Lane 2: Mouse Small intestine tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human HUVEC cell lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human 293T cell lysates, Primary: Anti-TSPAN8 (orb100339) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kDa, Observed band size: 35 kDa. |
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