CYPA Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB100498
- Images (6)
| Article Name: | CYPA Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB100498 |
| Supplier Catalog Number: | orb100498 |
| Alternative Catalog Number: | BYT-ORB100498-50,BYT-ORB100498-100,BYT-ORB100498-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Cyclophilin A. (101-165/165aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CYPA, CYPH, HEL-S-69p, Cphn, CyP-18, SP18, CYCA, CyP-A, p1B15, p31, PPIA_HUMAN, PPIA, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, 5.2.1.8, PPIA_MOUSE, PPIA_RAT, |
| CYPA Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 18 kDa |
| UniProt: | P62937 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PPIA |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CYPA antibody (orb100498), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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Sample: Cerebrum (Rat) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CYPA (orb100498) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 17/18 kD. |
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Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Lymph node tissue lysates, Lane 3: Mouse Lung tissue lysates, Lane 4: Mouse NIH/3T3 cell lysates, Lane 5: Rat Thymus tissue lysates, Lane 6: Rat Lymph node tissue lysates, Lane 7: Rat Lung tissue lysates, Lane 8: Human Hela cell lysates, Lane 9: Human PANC-1 cell lysates, Lane 10: Human Molt-4 cell lysates, Lane 11: Human HUVEC cell lysates, Lane 12: Human THP-1 cell lysates, Primary: Anti-CYPA (orb100498) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kDa, Observed band size: 18 kDa. |
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Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Primary: Anti-CYPA (orb100498) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 18 kD. |
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Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (orb100498) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (orb100498) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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