VHL Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB100644
- Images (6)
| Article Name: | VHL Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB100644 |
| Supplier Catalog Number: | orb100644 |
| Alternative Catalog Number: | BYT-ORB100644-50,BYT-ORB100644-100,BYT-ORB100644-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human VHL (101-200/213aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | HRCA1, RCA1, VHL1, pVHL, Vhlh, VHL_HUMAN, VHL, Protein G7, VHL_MOUSE, |
| VHL Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 24 kDa |
| UniProt: | P40337 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | VHL |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VHLH) Polyclonal Antibody, Unconjugated (orb100644) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VHL) Polyclonal Antibody, Unconjugated (orb100644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VHL) Polyclonal Antibody, Unconjugated (orb100644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VHLH) Polyclonal Antibody, Unconjugated (orb100644) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (VHL) Polyclonal Antibody, Unconjugated (orb100644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Rat Kidney tissue lysates, Primary: Anti-VHL (orb100644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 30 kDa. |
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