PKM2 Mouse Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB101937
- Images (6)
| Article Name: | PKM2 Mouse Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB101937 |
| Supplier Catalog Number: | orb101937 |
| Alternative Catalog Number: | BYT-ORB101937-50,BYT-ORB101937-100,BYT-ORB101937-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PKM2 (51-150/531aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58, Pk-2, Pk-3, PK, PKM12, KPYM_HUMAN, PKM, Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1 (THBP1), Tumor M2-PK, Tyrosine-protein kinase PKM2, 2.7.1.40, PK2, KPYM_MOUSE, Pykm, KPYM_RAT, |
| PKM2 Mouse Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 58 kDa |
| UniProt: | P14618 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PKM |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (orb101937) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Jurkat Cell (Human) Lysate at 40 ug, Primary: Anti-PKM2 (orb101937) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD. |
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Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: U87MG (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-PKM2 (orb101937) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
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Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-PKM2 (orb101937) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD. |
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Tissue/cell: Human nasopharyngeal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (orb101937) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (orb101937) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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