Caspase-9 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10243
- Images (7)
| Article Name: | Caspase-9 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10243 |
| Supplier Catalog Number: | orb10243 |
| Alternative Catalog Number: | BYT-ORB10243-50,BYT-ORB10243-100,BYT-ORB10243-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Caspase-9 subunit p35 (271-314/416aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, CASP-9, Caspase-9, Casp-9-CTD, Casp9_v1, CASP9_HUMAN, CASP9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3 (APAF-3), ICE-like apoptotic protease 6 (ICE-LAP6), 3.4.22.62, Q4FJK5_MOUSE, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, Q920G4_RAT, |
| Caspase-9 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 35/50 kDa |
| UniProt: | P55211 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CASP9 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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Blank control: K562 (blue). Primary Antibody: Rabbit Anti-caspase-9 antibody (orb10243, Green), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 80% methanol (5 min) and and then permeabilized with 0.01M PBS-Tween for 20 min. Primary antibody (orb10243, 1 µg/1x10 6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (30 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20000 events was performed. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Caspase-9) polyclonal Antibody, Unconjugated (orb10243) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (orb10243) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (orb10243) at 1:800 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (orb10243) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (orb10243) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Urinary bladder (Mouse) Lysate at 40 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Caspase-9 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46-513537 kD, Observed band size: 35 kD. |
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