CXCR4 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10305
- Images (6)
| Article Name: | CXCR4 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10305 |
| Supplier Catalog Number: | orb10305 |
| Alternative Catalog Number: | BYT-ORB10305-50,BYT-ORB10305-100,BYT-ORB10305-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from the middle of human CXCR4 (1-38/352aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1, CXC-R4, CXCR-4, Cmkar4, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo, CXCR4_HUMAN, CXCR4, Fusin, Leukocyte-derived seven transmembrane domain receptor (LESTR), Lipopolysaccharide-associated protein 3 (LAP-3 | LPS-associated protein 3), Stromal cell-derived factor 1 receptor (SDF-1 receptor), CXCR4_MOUSE, Pre-B-cell-derived chemokine receptor (PB-CKR), CXCR4_RAT, |
| CXCR4 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 40 kDa |
| UniProt: | P61073 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CXCR4 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA (green). |
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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CXCR4 antibody (orb10305), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10305, 1 µg/1x10 6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed. |
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Sample: Brain (Rat) Lysate at 30 ug, Heart (Rat) Lysate at 30 ug, Primary: Anti-CXCR4 (orb10305) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD. |
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Sample: Lane 1: Blood cell (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Lane 3: Bone (Rat) Lysate at 40 ug, Lane 4: Spleen (Rat) Lysate at 40 ug, Primary: Anti-CXCR4 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 44 kD. |
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Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CXCR4 (orb10305) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD. |
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Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD184/CXCR4 Polyclonal Antibody, Unconjugated (orb10305) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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