PLEKHO1 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10417
- Images (7)
| Article Name: | PLEKHO1 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10417 |
| Supplier Catalog Number: | orb10417 |
| Alternative Catalog Number: | BYT-ORB10417-50,BYT-ORB10417-100,BYT-ORB10417-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PLEKHO1 (51-170/409aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CKIP-1, CKIP1, JBP, OC120, 2810052M02Rik, JZA-20, Jza2, RGD1311487, PKHO1_HUMAN, PLEKHO1, PH domain-containing family O member 1, C-Jun-binding protein (JBP), Casein kinase 2-interacting protein 1 (CK2-interacting protein 1 | CKIP-1), Osteoclast maturation-associated gene 120 protein, PKHO1_MOUSE, PKHO1_RAT, |
| PLEKHO1 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 45 kDa |
| UniProt: | Q53GL0 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PLEKHO1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-PLEKHO1 (orb10417) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CKIP-1 Polyclonal Antibody, Unconjugated (orb10417) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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