GDNFRA Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10711
- Images (6)
| Article Name: | GDNFRA Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10711 |
| Supplier Catalog Number: | orb10711 |
| Alternative Catalog Number: | BYT-ORB10711-50,BYT-ORB10711-100,BYT-ORB10711-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human GFR Alpha-1 (351-429/465aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | GDNFR, GDNFR-alpha-1, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, RHDA4, TRNR1, GFRA1_HUMAN, GFRA1, GDNF receptor alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, GFRA1_MOUSE, GFRA1_RAT, |
| GDNFRA Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 44 kDa |
| UniProt: | P56159 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GFRA1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (orb10711) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human MOLT4 cell lysates, Lane 4: Human A549 cell lysates, Primary: Anti-GDNFRA (orb10711) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 47 kDa. |
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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-GDNFRA (orb10711) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 47 kDa. |
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Tissue/Cell: Human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated (orb10711) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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