GRP75 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10746
- Images (7)
| Article Name: | GRP75 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10746 |
| Supplier Catalog Number: | orb10746 |
| Alternative Catalog Number: | BYT-ORB10746-50,BYT-ORB10746-100,BYT-ORB10746-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human GRP75 (561-679/679aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | HSPA9_HUMAN, HSPA9, 75 kDa glucose-regulated protein (GRP-75), Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin (MOT), Peptide-binding protein 74 (PBP74), 3.6.4.10, GRP75, HSPA9B, mt-HSP70, HSPA9_MOUSE, Mortalin, p66 MOT, Hsp74, Hspa9a, HSPA9_RAT, mtHSP70, |
| GRP75 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 70 kDa |
| UniProt: | P38646 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | HSPA9 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Liver tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Cerebrum tissue lysates, Lane 7: Rat Pancreas tissue lysates, Lane 8: Human K562 cell lysates, Lane 9: Human MCF-7 cell lysates, Lane 10: Human Jurkat cell lysates, Lane 11: Human HeLa cell lysates, Lane 12: Human HepG2 cell lysates, Primary: Anti-GRP75 (orb10746) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kDa, Observed band size: 72 kDa. |
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