Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.6 kDa. The protein migrates as 57-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Mouse IFN-alpha / beta R1, His Tag (Cat. No. orb1087552) immobilized on CM5 Chip can bind Mouse IFN-alpha 1, His Tag (Cat. No. orb735042) with an affinity constant of 2.96 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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