IL6ST/CD130 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB10914
- Images (7)
| Article Name: | IL6ST/CD130 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB10914 |
| Supplier Catalog Number: | orb10914 |
| Alternative Catalog Number: | BYT-ORB10914-50,BYT-ORB10914-100,BYT-ORB10914-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human IL6R beta (651-750/917aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD130, CDW130, GP130, HIES4, HIES4A, HIES4B, IL-6RB, IMD94, STWS2, sGP130, 5133400A03Rik, D13Ertd699e, IL6RB_HUMAN, IL6ST, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, Interleukin-6 signal transducer, Membrane glycoprotein 130 (gp130), Oncostatin-M receptor subunit alpha, IL6RB_MOUSE, |
| IL6ST/CD130 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 99 kDa |
| UniProt: | P40189 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | IL6ST |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CD34 antibody (orb10914), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Antibody (orb10914, 1 µg/1x10 6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of orb10914 at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD130) Polyclonal Antibody, Unconjugated (orb10914) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD130) Polyclonal Antibody, Unconjugated (orb10914) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lung (Mouse) Lysate at 30 ug, Primary: Anti-CD130 (orb10914) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 99 kD, Observed band size: 130 kD. |
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Sample: lung (mouse) Lysate at 40 ug, Primary: Anti-CD130 (orb10914) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 99kD, Observed band size: 130 kD. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-IL-6R Beta/CD130/gp130 Polyclonal Antibody, Unconjugated (orb10914) 1:300, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Tissue/Cell: Human endometrium carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-IL-6R Beta/CD130/gp130 Polyclonal Antibody, Unconjugated (orb10914) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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