Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunoprecipitation, 0.5-2 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x
Flow Cytometry analysis of CACO-2 cells using anti-P38 Alpha/MAPK14 antibody. Overlay histogram showing CACO-2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P38 Alpha/MAPK14 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody. p38 alpha/MAPK14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody. p38 alpha/MAPK14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody. p38 alpha/MAPK14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-p38 alpha/MAPK14 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Immunoprecipitating p38 alpha/MAPK14 in Jurkat whole cell lysate. Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody, Lane 1: Jurkat whole cell lysates (30 ug), Lane 2: Rabbit control IgG instead of anti-p38 alpha/MAPK14 antibody in Jurkat whole cell lysate, Lane 3: anti-p38 alpha/MAPK14 antibody (2 µg) + Jurkat whole cell lysate (500 µg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p38 alpha/MAPK14 antigen affinity purified polyclonal antibody at a dilution of 0.5 µg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for p38 alpha/MAPK14 at approximately 38 kDa. The expected band size for p38 alpha/MAPK14 is at 41 kDa.
Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat he
Western blot analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody.
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