Biological Activity: Immobilized Cynomolgus Complement C3, His Tag at 1 µg/mL (100 µL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested). Application Notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement component 3 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 71.3 kDa (beta chain) and 113.0 kDa (alpha chain). The protein migrates as 64-68 kDa (beta chain) and 100-120 kDa (alpha chain) and 160-180 kDa (intact Complement component 3) under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Cynomolgus Complement C3, His Tag (Cat. No. orb1101181) at 1 µg/mL (100 µL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested).
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