NFKB1 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11124
- Images (7)
| Article Name: | NFKB1 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11124 |
| Supplier Catalog Number: | orb11124 |
| Alternative Catalog Number: | BYT-ORB11124-50,BYT-ORB11124-100,BYT-ORB11124-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Nuclear factor NF-kappa-B p50 subunit (51-100/968aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB, NF-kappaB1, p105, p50, p50/p105, NFKB1_HUMAN, NFKB1, DNA-binding factor KBF1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, NFKB1_MOUSE, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1_RAT, |
| NFKB1 Rabbit Polyclonal Antibody |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NFkB p50) Polyclonal Antibody, Unconjugated (orb11124) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Brain (Mouse) Lysate at 30 ug, Primary: Anti-NFkB p105/p50 (orb11124) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 48/105 kD, Observed band size: 50 kD. |
|
|
Sample: Lane 1: Human Raji cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-NFKB1 (orb11124) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48/105 kDa, Observed band size: 135 kDa. |
|
|
Sample: Lung (Mouse) lysate at 30 ug, Primary: Anti-NFkB p105/p50 (orb11124) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 48/105 kD, Observed band size: 110 kD. |
Product Guarantee and Expert Support
