PDCD4 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11247
- Images (5)
| Article Name: | PDCD4 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11247 |
| Supplier Catalog Number: | orb11247 |
| Alternative Catalog Number: | BYT-ORB11247-50,BYT-ORB11247-100,BYT-ORB11247-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PDCD4 (1-100/469aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | H731, D19Ucla1, Ma3, Tis, Dug, PDCD4_HUMAN, PDCD4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, PDCD4_MOUSE, Protein MA-3, Topoisomerase-inhibitor suppressed protein, PDCD4_RAT, Death up-regulated gene protein, |
| PDCD4 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 52 kDa |
| UniProt: | Q53EL6 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PDCD4 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
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Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-PDCD4 antibody (orb11247), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PDCD4) polyclonal Antibody, Unconjugated (orb11247) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Sample: Lane 1: Kidney (Rat) Lysate at 30 ug, Lane 2: Colon carcinoma (Mouse) Lysate at 30 ug, Primary: Anti-PDCD4 (orb11247) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 50kD, Observed band size: 50kD. |
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Tissue/Cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PDCD4 Polyclonal Antibody, Unconjugated (orb11247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PDCD4 Polyclonal Antibody, Unconjugated (orb11247) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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