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Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (orb11263) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Liver (Rat) Lysate at 40 ug, Lane 3: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-SLC15A1 (orb11263) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 83 kD. |