Phospho-FSCN1 (Ser39) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11266
- Images (6)
| Article Name: | Phospho-FSCN1 (Ser39) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11266 |
| Supplier Catalog Number: | orb11266 |
| Alternative Catalog Number: | BYT-ORB11266-50,BYT-ORB11266-100,BYT-ORB11266-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39 AS(p-S)LK |
| Conjugation: | Unconjugated |
| Alternative Names: | FSCN1 (p-S39), p-FSCN1, phospho-FSCN1, FAN1, HSN, SNL, p55, fascin-1, Fascin, FSCN1_HUMAN, FSCN1, 55 kDa actin-bundling protein, Singed-like protein, FSCN1_MOUSE, FSCN1_RAT, |
| Phospho-FSCN1 (Ser39) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 55 kDa |
| UniProt: | Q16658 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FSCN1 |
| Application Dilute: | WB=1:500-2000 |
| Application Notes: | Modification: Phosphorylated |
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A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (orb11266) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Blank control: U937 (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 3 µl in 100 µl 1X PBS containing 0.5% BSA (green). |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FSCN1 (Ser39)) Polyclonal Antibody, Unconjugated (orb11266) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Spleen tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Spleen tissue lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human K562 cell lysates, Lane 9: Human SH-SY5Y cell lysates, Lane 10: Human U-2os cell lysates, Primary: Anti-phospho-FSCN1 (Ser39) (orb11266) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 51 kDa. |
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Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-FSCN1 (Ser39) (orb11266) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD. |
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Tissue/Cell: musle of mouse embryo, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-FSCN1 (Ser39) Polyclonal Antibody, Unconjugated (orb11266) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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