Prohibitin Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB11270
Article Name: Prohibitin Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB11270
Supplier Catalog Number: orb11270
Alternative Catalog Number: BYT-ORB11270-50,BYT-ORB11270-100,BYT-ORB11270-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Prohibitin (171-272/272aa)
Conjugation: Unconjugated
Alternative Names: BAP32, HEL-215, HEL-S-54e, PHB, PHB1_HUMAN, PHB1, PHB1_MOUSE, B-cell receptor-associated protein 32 (BAP 32), PHB1_RAT,
Prohibitin Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 30 kDa
UniProt: P35232
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: PHB
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/test
HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (orb11270) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Sample: A431 (Human) Cell Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.
Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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