PIK3CA Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11272
- Images (6)
| Article Name: | PIK3CA Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11272 |
| Supplier Catalog Number: | orb11272 |
| Alternative Catalog Number: | BYT-ORB11272-50,BYT-ORB11272-100,BYT-ORB11272-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PI3KCA (961-1068/1068aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha, PK3CA_HUMAN, PIK3CA, PI3-kinase subunit alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha | p110alpha), Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, 2.7.1.137, 2.7.1.153, |
| PIK3CA Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 124 kDa |
| UniProt: | P42336 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PIK3CA |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PIK3CA) polyclonal Antibody, Unconjugated (orb11272) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (orb11272) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (orb11272) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-PIK3CA (orb11272) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117kD, Observed band size: 120kD. |
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Sample: Lane 1: Human SW480 cell lysates, Lane 2: Human Molt-4 cell lysates, Lane 3: Human HL60 cell lysates, Primary: Anti-PIK3CA (orb11272) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 110 kD. |
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Sample: SW480 (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-PIK3CA (orb11272) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 124 kD. |
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