Cell culture (A549), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.
Cell culture (U251), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (semaphorin 3F) Polyclonal Antibody, Unconjugated (orb11360) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Sema3F Polyclonal Antibody, Unconjugated (orb11360) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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