E.coli-derived human Sorbitol Dehydrogenase/SORD recombinant protein (Position: N8-P357).
Conjugation:
Unconjugated
Alternative Names:
L iditol 2 dehydrogenase, SDH, Sorbitol dehydrogenase, SORD, SORD1
Anti-Sorbitol Dehydrogenase/SORD Antibody (monoclonal, 12B10G2). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Clonality:
Monoclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Form:
Lyophilized
Target:
Sorbitol dehydrogenase
Application Dilute:
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Rat Flow Cytometry (Fixed), 1-3 µg/1x10 6 cells, Human
Flow Cytometry analysis of U20S cells using anti-Sorbitol Dehydrogenase/SORD antibody. Overlay histogram showing U20S cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Sorbitol Dehydrogenase/SORD Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-mouse IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
Western blot analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue tissue lysates, Lane 4: rat kidney tissue ly
IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorb
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